Shimadzu Spd20a User Manual

| Symptom | Probable Cause | Corrective Action | |---------|----------------|--------------------| | High baseline noise | Air bubbles in cell | Flush with degassed mobile phase or remove cell and rinse. | | Negative peaks | Sample absorbs less than mobile phase at set wavelength | Change wavelength or check mobile phase UV cutoff. | | Lamp fails to ignite | Lamp expired or power supply issue | Check lamp hours; replace D2 lamp. | | No signal at high sensitivity | Flow cell window dirty | Clean cell with 1M HNO₃, then water, then methanol. | | Wavelength error | Stepper motor failure or filter stuck | Run Wavelength Calibration from service menu (qualified personnel only). |

: Perform a "Zero" or "Autozero" once the mobile phase is flowing and the baseline has stabilized. Routine Maintenance shimadzu spd20a user manual

Warm-up: The D2 lamp requires at least 30 minutes to stabilize before starting an analysis. Operating before the lamp is warm can result in baseline drift. Operating Procedures | Symptom | Probable Cause | Corrective Action

The Shimadzu SPD-20A UV-Vis detector is a powerful tool for chromatographic analysis, offering high sensitivity and reliability. By understanding its features, proper operation, and maintenance, users can ensure optimal performance and extend the lifespan of the device. Always refer to the official user manual for detailed instructions and specifications. | | No signal at high sensitivity |